Detection of SARS-CoV-2 spike protein D614G mutation by qPCR-HRM analysis.

ABSTRACT:

Objectives

Monitoring the spread of the G614 in specific locations is critical as this variant is highly transmissible and can trigger the emergence of other mutations. Therefore, a rapid and accurate method that can reliably detect the D614G mutation will be beneficial. This study aims to analyze the potential use of the two-step Reverse Transcriptase quantitative polymerase chain reaction - high resolution melting analysis (RT-qPCR-HRM) to detect a specific mutation in the SARS-CoV-2 genome.

Methods

Six SARS-CoV-2 RNA samples were synthesized into cDNA and analyzed with the qPCR-HRM method in order to detect the D614G mutation in Spike protein of SARS-CoV-2. The primers are designed to target the specific Spike region containing the D614G mutation. The qPCR-HRM anal

SUBMITTER: Gazali FM 

PROVIDER: S-EPMC8420086 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature