Cross-neutralization of RBD mutant strains of SARS-CoV-2 by convalescent patient derived antibodies.

ABSTRACT:

Background

The emergence of COVID-19 pandemic resulted in an urgent need for the development of therapeutic interventions. Of which, neutralizing antibodies play a crucial role in the prevention and resolution of viral infection.

Methods

We generated antibody libraries from 18 different COVID-19 recovered patients and screened neutralizing antibodies to SARS-CoV-2 and its mutants. After 3 rounds of panning, 456 positive phage clones were obtained with high affinity to RBD (receptor binding domain). Clones were then reconstituted into whole human IgG for epitope binning assay and all 19 IgG were classified into 6 different epitope groups or Bins.

Results

Although all antibodies were found to bind RBD, the antibodies in Bin2 had superior inhibitory ability of the intera

SUBMITTER: Lou Y 

PROVIDER: S-EPMC8420279 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature