IFN-γ⁺ cell response and IFN-γ release concordance after in vitro SARS-CoV-2 stimulation.

ABSTRACT:

Background

Healthcare workers (HCWs) are at increased risk since they are directly exposed to SARS-CoV-2-infected patients, and nevertheless, some remain without the development of anti-SARS-CoV-2 antibodies or related symptoms, suggesting less susceptibility to the infection.

Methods

This cross-sectional, case-control study aimed to compare SARS-CoV-2 T-cell response by two different technologies, the analysis of IFN-γ⁺ CD8⁺ /CD4⁺ T cells by flow cytometry and the quantification of IFN-γ release by ELISA-related assay (without cell discrimination), both after SARS-CoV-2 stimulation among uninfected and convalescent HCWs.

Results

A high proportion of uninfected HCWs (53.8%) had pre-existing IFN-γ⁺ CD8 T-cell response after s

SUBMITTER: Vallejo A 

PROVIDER: S-EPMC8420579 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature