In vitro generation of self-renewing human intestinal epithelia over planar and shaped collagen hydrogels.

ABSTRACT: The large intestine, with its array of crypts lining the epithelium and diverse luminal contents, regulates homeostasis throughout the body. In vitro crypts formed from primary human intestinal epithelial stem cells on a 3D shaped hydrogel scaffold replicate the functional and architectural features of in vivo crypts. Collagen scaffolding assembly methods are provided, along with the microfabrication and soft lithography protocols necessary to shape these hydrogels to match the dimensions and density of in vivo crypts. In addition, stem-cell scale-up protocols are provided so that even ultrasmall primary samples can be used as starting material. Initially, these cells are seeded as a proliferative monolayer over the shaped scaffold and cultured as stem/proliferative cells to expand them an

SUBMITTER: Hinman SS 

PROVIDER: S-EPMC8420814 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature