High-Resolution Microscopy to Learn the Nuclear Organization of the Living Yeast Cells.

ABSTRACT: The spatial organization of the nucleus is a key determinant in all genome activities. However, the accurate measurement of the nuclear organization is still technically challenging. Here, the technology NucQuant we created previously was utilized to detect the variation of the nuclear organization, including the heterogeneity of the nuclear geometry, the change of the NPC distribution along different cell cycle stages during interphase, and the organization of the nucleolus. The results confirmed that not only the growth rate and the NPC distribution are influenced by the carbon source; the nuclear shape is also impacted by the carbon source. The nuclei lost their spherical geometry gradually when the cell was cultured from the most to a less favorable carbon source. We also discovered th

SUBMITTER: Wang R 

PROVIDER: S-EPMC8421178 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature