Differential roles of RIG-I like receptors in SARS-CoV-2 infection.

ABSTRACT: Retinoic acid-inducible gene I (RIG-I) and melanoma differentiation-associated protein 5 (MDA5) sense viral RNA and activate antiviral immune responses. Herein we investigate their functions in human epithelial cells, the primary and initial target of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). A deficiency in MDA5, RIG-I or mitochondrial antiviral signaling protein (MAVS) enhanced viral replication. The expression of the type I/III interferon (IFN) during infection was impaired in MDA5^-/- and MAVS^-/-, but not in RIG-I^-/-, when compared to wild type (WT) cells. The mRNA level of full-length angiotensin-converting enzyme 2 (ACE2), the cellular entry receptor for SARS-CoV-2, was ~ 2.5-fold higher in RIG-I^-/- than WT cells. These d

SUBMITTER: Yang DM 

PROVIDER: S-EPMC8421188 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature