EIF4G is retained on ribosomes elongating and terminating on short upstream ORFs to control reinitiation in yeast.

ABSTRACT: Translation reinitiation is a gene-specific translational control mechanism. It is characterized by the ability of short upstream ORFs to prevent full ribosomal recycling and allow the post-termination 40S subunit to resume traversing downstream for the next initiation event. It is well known that variable transcript-specific features of various uORFs and their prospective interactions with initiation factors lend them an unequivocal regulatory potential. Here, we investigated the proposed role of the major initiation scaffold protein eIF4G in reinitiation and its prospective interactions with uORF's cis-acting features in yeast. In analogy to the eIF3 complex, we found that eIF4G and eIF4A but not eIF4E (all constituting the eIF4F complex) are preferentially retained on ribosomes elongati

SUBMITTER: Mohammad MP 

PROVIDER: S-EPMC8421214 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature