C-kit2 G-quadruplex stabilized via a covalent probe: exploring G-quartet asymmetry.

ABSTRACT: Several sequences forming G-quadruplex are highly conserved in regulatory regions of genomes of different organisms and affect various biological processes like gene expression. Diverse G-quadruplex properties can be modulated via their interaction with small polyaromatic molecules such as pyrene. To investigate how pyrene interacts with G-rich DNAs, we incorporated deoxyuridine nucleotide(s) with a covalently attached pyrene moiety (Upy) into a model system that forms parallel G-quadruplex structures. We individually substituted terminal positions and positions in the pentaloop of the c-kit2 sequence originating from the KIT proto-oncogene with Upy and performed a detailed NMR structural study accompanied with molecular dynamic simulations. Our results showed that incorporation into the p

SUBMITTER: Peterkova K 

PROVIDER: S-EPMC8421218 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature