Label-free concurrent 5-modal microscopy (Co5M) resolves unknown spatio-temporal processes in wound healing.

ABSTRACT: The non-invasive investigation of multiple biological processes remains a methodological challenge as it requires capturing different contrast mechanisms, usually not available with any single modality. Intravital microscopy has played a key role in dynamically studying biological morphology and function, but it is generally limited to resolving a small number of contrasts, typically generated by the use of transgenic labels, disturbing the biological system. We introduce concurrent 5-modal microscopy (Co5M), illustrating a new concept for label-free in vivo observations by simultaneously capturing optoacoustic, two-photon excitation fluorescence, second and third harmonic generation, and brightfield contrast. We apply Co5M to non-invasively visualize multiple wound healing biomarkers and

SUBMITTER: Seeger M 

PROVIDER: S-EPMC8421396 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature