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Longitudinal characterization of multispecies microbial populations recovered from spaceflight potable water.

ABSTRACT: While sequencing technologies have revolutionized our knowledge of microbial diversity, little is known about the dynamic emergent phenotypes that arise within the context of mixed-species populations, which are not fully predicted using sequencing technologies alone. The International Space Station (ISS) is an isolated, closed human habitat that can be harnessed for cross-sectional and longitudinal functional microbiome studies. Using NASA-archived microbial isolates collected from the ISS potable water system over several years, we profiled five phenotypes: antibiotic resistance, metabolism, hemolysis, and biofilm structure/composition of individual or multispecies communities, which represent characteristics that could negatively impact astronaut health and life-support systems. Data revealed a temporal dependence on interactive behaviors, suggesting possible microbial adaptation over time within the ecosystem. This study represents one of the most extensive phenotypic characterization of ISS potable water microbiota with implications for microbial risk assessments of water systems in built environments in space and on Earth.

SUBMITTER: Yang J

PROVIDER: S-EPMC8421509 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature

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Longitudinal characterization of multispecies microbial populations recovered from spaceflight potable water.

Yang Jiseon J Barrila Jennifer J Mark Ott C C King Olivia O Bruce Rebekah R McLean Robert J C RJC Nickerson Cheryl A CA

NPJ biofilms and microbiomes 20210906 1

While sequencing technologies have revolutionized our knowledge of microbial diversity, little is known about the dynamic emergent phenotypes that arise within the context of mixed-species populations, which are not fully predicted using sequencing technologies alone. The International Space Station (ISS) is an isolated, closed human habitat that can be harnessed for cross-sectional and longitudinal functional microbiome studies. Using NASA-archived microbial isolates collected from the ISS pota ...[more]

PMID: 34489467

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Dataset Information

Longitudinal characterization of multispecies microbial populations recovered from spaceflight potable water.

Publications

Longitudinal characterization of multispecies microbial populations recovered from spaceflight potable water.

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