Proteomic analysis exploring the mechanism of bladder fibrosis induced by ketamine using a rat model.

ABSTRACT:

Background

Long-term abuse of ketamine can cause irreversible bladder fibrosis, but the mechanism of bladder fibrosis is still under investigation. This study aims to explore the mechanism of bladder fibrosis utilizing proteomic analysis in a rat model.

Methods

After building a rat model, hematoxylin and eosin (HE) staining, Masson's trichrome staining, and western blotting (WB) of collagen I were used to assess bladder pathology and fibrosis in a rat model. Next, protein expression changes in the rat bladder by proteomic technology were quantitatively detected, and reverse transcription-polymerase chain reaction (RT-PCR) and WB were used to verify the expression of proteins. Bioinformatic techniques and functional analysis were also performed.

Results

Compared to the

SUBMITTER: Zhu Q 

PROVIDER: S-EPMC8421841 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature