XPA serves as an autophagy and apoptosis inducer by suppressing hepatocellular carcinoma in a PI3K/Akt/mTOR dependent manner.

ABSTRACT:

Background

To explore the potential biological function of XPA (Xeroderma pigmentosum group A) in hepatic neoplasms and the underlying molecular mechanisms.

Methods

Liver cells were used as experimental models to establish HCC (hepatocellular carcinoma) in vitro. Protein extractions were subjected to Western blotting to detect the proteins expression. The lentivirus transfection efficiency was confirmed by Western blot and RT-qPCR, Tunnel staining was used to detect apoptosis, and Transwell assays were used to observe cell migration and invasion. Cell proliferation was detected with colony formation and CCK-8 (cell counting kit-8) assays.

Results

XPA expression was obviously lower in HCC tissue and liver cancer cell lines. XPA overexpression induced autophagy and apop

SUBMITTER: Deng Y 

PROVIDER: S-EPMC8421913 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature