Ozone induces BEL7402 cell apoptosis by increasing reactive oxygen species production and activating JNK.

ABSTRACT:

Background

Oxidative stress is an important factor in the modulation of both tumorigenesis and anticancer responses. Ozone (O₃) is a strong oxidant that causes redox reactions and exerts anticancer effects in various types of cancer cells. However, the pathways involved in O₃-induced cell death are not well understood.

Methods

In vitro human hepatocellular carcinoma (HCC) BEL7402 cells were treated with various O₃ concentrations to evaluate O₃ cytotoxicity by Cell Counting Kit-8 (CCK-8) assay and flow cytometry. The regulatory mechanisms were analyzed by western blot analysis. In vivo, an HCC model was established to evaluate the inhibition of HCC with O₃ treatment.

Results

In vitro cells trea

SUBMITTER: Tang S 

PROVIDER: S-EPMC8421928 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature