Analysis of the retinal capillary plexus layers in a murine model with diabetic retinopathy: effect of intravitreal injection of human CD34⁺ bone marrow stem cells.

ABSTRACT:

Background

Diabetic retinopathy is a retinal vasculopathy involving all three retinal capillary plexus layers. Since human CD34⁺ bone marrow stem cells (BMSCs) have the potential to promote revascularization of ischemic tissue, this study tests the hypothesis that intravitreal injection of human CD34⁺ BMSCs can have protective effects on all layers of the retinal vasculature in eyes with diabetic retinopathy.

Methods

Streptozotocin (STZ)-induced diabetic mice were injected intravitreally with 50,000 human CD34⁺ BMSCs or phosphate-buffered saline (PBS) into the right eye. Systemic immunosuppression with rapamycin and tacrolimus was started 5 days before the injection and maintained for study duration to prevent rejection of human cells. All mi

SUBMITTER: Cheung KW 

PROVIDER: S-EPMC8421965 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature