The RNA binding protein hnRNPK protects against adriamycin-induced podocyte injury.

ABSTRACT:

Background

Podocytes maintain the integrity of the glomerular filtration barrier and serve as the final barrier to protein loss. Podocyte injury may induce severe apoptosis, which can result in serious kidney damage and disease. Therefore, it is necessary to explore how podocyte injury can be prevented and to thereby discover a feasible therapy for kidney disease. However, the mechanism of podocyte injury is still unclear.

Methods

The mRNA and protein expression levels of synaptopodin and nephrin in MPC5 podocytes with adriamycin (ADR)-induced injury were detected by quantitative real-time PCR and western blot. The expression levels of heterogeneous nucleotide protein K (hnRNPK), caspase-3, Bax, and Bcl-2 protein in cells and tissues were measured using western blot. Prolife

SUBMITTER: Zhao S 

PROVIDER: S-EPMC8422093 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature