Circulating microRNAs as biomarkers in bile-derived exosomes of cholangiocarcinoma.

ABSTRACT:

Purpose

In this pilot study, using next-generation sequencing and integrated messenger RNA (mRNA) sequencing, we investigated circulating microRNA (miRNA) expression profiling from bile-derived exosomes to identify dysregulated miRNA signatures and oncogenic pathways and determine their effects on targeted mRNAs in cholangiocarcinoma (CCA). Moreover, we explored the possibility that genetic analysis using bile-derived exosomes may replace gene analysis using tissue.

Methods

Bile was collected from a patient with perihilar CCA before curative resection. As a control, bile was collected from a patient with a common bile duct stone. Exosomes were isolated from the bile, and we performed next-generation miRNA sequencing using isolated exosomes. To evaluate miRNA-mRNA interaction

SUBMITTER: Han JY 

PROVIDER: S-EPMC8424434 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature