Indirect podocyte injury manifested in a partial podocytectomy mouse model.

ABSTRACT: In progressive glomerular diseases, segmental podocyte injury often expands, leading to global glomerulosclerosis by unclear mechanisms. To study the expansion of podocyte injury, we established a new mosaic mouse model in which a fraction of podocytes express human (h)CD25 and can be injured by the immunotoxin LMB2. hCD25⁺ and hCD25^- podocytes were designed to express tdTomato and enhanced green fluorescent protein (EGFP), respectively, which enabled cell sorting analysis of podocytes. After the injection of LMB2, mosaic mice developed proteinuria and glomerulosclerosis. Not only tdTomato⁺ podocytes but also EGFP⁺ podocytes were decreased in number and showed damage, as evidenced by a decrease in nephrin and an increase in desmin at both protein

SUBMITTER: Okabe M 

PROVIDER: S-EPMC8424557 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature