L-WNK1 is required for BK channel activation in intercalated cells.

ABSTRACT: Large-conductance K⁺ (BK) channels expressed in intercalated cells (ICs) in the aldosterone-sensitive distal nephron (ASDN) mediate flow-induced K⁺ secretion. In the ASDN of mice and rabbits, IC BK channel expression and activity increase with a high-K⁺ diet. In cell culture, the long isoform of with-no-lysine kinase 1 (L-WNK1) increases BK channel expression and activity. Apical L-WNK1 expression is selectively enhanced in ICs in the ASDN of rabbits on a high-K⁺ diet, suggesting that L-WNK1 contributes to BK channel regulation by dietary K⁺. We examined the role of IC L-WNK1 expression in enhancing BK channel activity in response to a high-K⁺ diet. Mice with IC-selective deletion of L-WNK1 (IC-L-WNK1-KO) and littermate cont

SUBMITTER: Ray EC 

PROVIDER: S-EPMC8424664 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature