Construction of a la carte QconCAT protein standards for multiplexed quantification of user-specified target proteins.

ABSTRACT:

Background

QconCATs are quantitative concatamers for proteomic applications that yield stoichiometric quantities of sets of stable isotope-labelled internal standards. However, changing a QconCAT design, for example, to replace poorly performing peptide standards has been a protracted process.

Results

We report a new approach to the assembly and construction of QconCATs, based on synthetic biology precepts of biobricks, making use of loop assembly to construct larger entities from individual biobricks. The basic building block (a Qbrick) is a segment of DNA that encodes two or more quantification peptides for a single protein, readily held in a repository as a library resource. These Qbricks are then assembled in a one tube ligation reaction that enforces the order of assemb

SUBMITTER: Johnson J 

PROVIDER: S-EPMC8425055 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature