MicroRNA regulation of the proliferation and apoptosis of Leydig cells in diabetes.

ABSTRACT:

Background

The number of patients with diabetes is increasing worldwide. Diabetic testicular damage can cause spermiogenesis disorders and sexual dysfunction. We thus explored the role of miRNAs in diabetic testicular damage, and revealed that they could serve as effective prevention and treatment therapeutic targets.

Methods

Streptozotocin (STZ) was used to generate a rat model of type 2 diabetes. Rat testicular tissues were used for miRNA and mRNA sequencing. Through bioinformatics analysis, we constructed an miRNA-mRNA diabetic testicular damage regulatory network and screened for key miRNAs. We also used Leydig cells to generate a diabetic cell model and detected the downstream target genes of miRNAs, secretion of testosterone, and proliferation and apoptotic levels to e

SUBMITTER: Hu L 

PROVIDER: S-EPMC8425090 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature