Identification of reference genes for gene expression studies among different developmental stages of murine hearts.

ABSTRACT:

Background

Real-time quantitative polymerase chain reaction (RT-qPCR) is a widely-used standard assay for assessing gene expression. RT-qPCR data requires reference genes for normalization to make the results comparable. Therefore, the selected reference gene should be highly stable in its expression throughout the experimental datasets. So far, reports about the optimal set of reference genes in murine left ventricle (LV) across embryonic and postnatal stages are few. The objective of our research was to identify the appropriate reference genes in murine LV among different developmental stages.

Methods

We investigated the gene expression profiles of 21 widely used housekeeping genes in murine LV from 7 different developmental stages (almost throughout the whole period of th

SUBMITTER: Ren J 

PROVIDER: S-EPMC8425138 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature