Analysis of electric cigarette liquid effect on mouse brain tumor growth through EGFR and ERK activation.

ABSTRACT:

Introduction

Recently, electric cigarettes with liquid (e-liquid) were introduced as an alternative to tobacco smoking. They were promoted as possible cessation aids and were considered to be potentially less harmful than traditional tobacco-based cigarettes. However, there is little information on the toxicants present in e-liquids and their possible carcinogenic effects.

Methods

Western blot analysis was performed to identify the protein levels of cancer progression related signal transducers. Patient-derived brain tumor cells (CSC2) were injected into mouse brains and tumor growth was then observed by performing magnetic resonance imaging (MRI) and hematoxylin and eosin (H&E) staining of the whole brain. Immunohistochemistry (IHC) staining and Immunofluorescence staining

SUBMITTER: Kwon HJ 

PROVIDER: S-EPMC8425573 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature