Serine Protease HTRA1 as a Novel Target Antigen in Primary Membranous Nephropathy.

ABSTRACT:

Background

Identification of target antigens PLA2R, THSD7A, NELL1, or Semaphorin-3B can explain the majority of cases of primary membranous nephropathy (MN). However, target antigens remain unidentified in 15%-20% of patients.

Methods

A multipronged approach, using traditional and modern technologies, converged on a novel target antigen, and capitalized on the temporal variation in autoantibody titer for biomarker discovery. Immunoblotting of human glomerular proteins followed by differential immunoprecipitation and mass spectrometric analysis was complemented by laser-capture microdissection followed by mass spectrometry, elution of immune complexes from renal biopsy specimen tissue, and autoimmune profiling on a protein fragment microarray.

Results

These approaches

SUBMITTER: Al-Rabadi LF 

PROVIDER: S-EPMC8425645 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature