3D Mapping Reveals a Complex and Transient Interstitial Matrix During Murine Kidney Development.

ABSTRACT:

Background

The extracellular matrix (ECM) is a network of proteins and glycosaminoglycans that provides structural and biochemical cues to cells. In the kidney, the ECM is critical for nephrogenesis; however, the dynamics of ECM composition and how it relates to 3D structure during development is unknown.

Methods

Using embryonic day 14.5 (E14.5), E18.5, postnatal day 3 (P3), and adult kidneys, we fractionated proteins based on differential solubilities, performed liquid chromatography-tandem mass spectrometry, and identified changes in ECM protein content (matrisome). Decellularized kidneys were stained for ECM proteins and imaged in 3D using confocal microscopy.

Results

We observed an increase in interstitial ECM that connects the stromal mesenchyme to the basement m

SUBMITTER: Lipp SN 

PROVIDER: S-EPMC8425666 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature