Circulating microRNAs from the mouse tibia fracture model reflect the signature from patients with complex regional pain syndrome.

ABSTRACT:

Introduction

Complex regional pain syndrome (CRPS) often results from an initial trauma that later produces a disproportionate amount of pain. The mechanisms underlying CRPS have been studied using a tibia fracture model (TFM) in rodents because this model closely mimics symptoms and has several molecular correlates observed in patients with CRPS.

Objective

Here, we determined whether the TFM has alterations in circulating microRNAs (miRNAs) and cytokines transported by small extracellular vesicles (sEVs) that faithfully model previously reported miRNA alterations from patients with CRPS.

Methods

We isolated and characterized serum-derived sEVs from mice 3 weeks after fracture when symptoms such as pain hypersensitivity develop. Whole-transcriptome profiling was used

SUBMITTER: Wickman JR 

PROVIDER: S-EPMC8425838 | biostudies-literature | 2021 Sep-Oct

REPOSITORIES: biostudies-literature