Bioactivity effects of extracellular matrix proteins on apical papilla cells.

ABSTRACT:

Background

Potent signaling agents stimulate and guide pulp tissue regeneration, especially in endodontic treatment of teeth with incomplete root formation.

Objective

This study evaluated the bioactive properties of low concentrations of extracellular matrix proteins on human apical papilla cells (hAPCs).

Methodology

Different concentrations (1, 5, and 10 µg/mL) of fibronectin (FN), laminin (LM), and type I collagen (COL) were applied to the bottom of non-treated wells of sterilized 96-well plates. Non-treated and pre-treated wells were used as negative (NC) and positive (PC) controls. After seeding the hAPCs (5×103 cells/well) on the different substrates, we assessed the following parameters: adhesion, proliferation, spreading, total collagen/type I collagen synthesi

SUBMITTER: Leite ML 

PROVIDER: S-EPMC8425894 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature