Intravital microscopy of tumor vessel morphology and function using a standard fluorescence microscope.

ABSTRACT:

Purpose

The purpose of the study was to demonstrate the performance and possible applications of an intravital microscopy assay using a standard fluorescence microscope.

Methods

Melanoma and pancreatic ductal adenocarcinoma xenografts were initiated in dorsal window chambers and subjected to repeated intravital microscopy. The entire tumor vasculature as well as the normal tissue surrounding the tumor was imaged simultaneously with high spatial and temporal resolution. Vascular morphology images were recorded by using transillumination, and vascular masks were produced to quantify vessel density, vessel diameter, vessel segment length, and vessel tortuosity. First-pass imaging movies were recorded after an intervenous injection of a fluorescent marker and were used to invest

SUBMITTER: Gaustad JV 

PROVIDER: S-EPMC8426228 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature