Highly tunable TetR-dependent target gene expression in the acetic acid bacterium Gluconobacter oxydans.

ABSTRACT: For the acetic acid bacterium (AAB) Gluconobacter oxydans only recently the first tight system for regulatable target gene expression became available based on the heterologous repressor-activator protein AraC from Escherichia coli and the target promoter P_araBAD. In this study, we tested pure repressor-based TetR- and LacI-dependent target gene expression in G. oxydans by applying the same plasmid backbone and construction principles that we have used successfully for the araC-P_araBAD system. When using a pBBR1MCS-5-based plasmid, the non-induced basal expression of the Tn10-based TetR-dependent expression system was extremely low. This allowed calculated induction ratios of up to more than 3500-fold with the fluorescence reporter protein mNeonGreen (mNG). The induct

SUBMITTER: Fricke PM 

PROVIDER: S-EPMC8426231 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature