Enhancement of lysine biosynthesis confers high-temperature stress tolerance to Escherichia coli cells.

ABSTRACT: Lysine, a nutritionally important amino acid, is involved in adaptation and tolerance to environmental stresses in various organisms. Previous studies reported that lysine accumulation occurs in response to stress and that lysine supplementation enhances stress tolerance; however, the effect of lysine biosynthesis enhancement on stress tolerance has yet to be elucidated. In this study, we confirmed that lysine supplementation to the culture medium increased intracellular lysine content and improved cell growth of Escherichia coli at high temperature (42.5 °C). Lysine-overproducing strains were then isolated from the lysine analogue S-adenosylmethionine-resistant mutants by conventional mutagenesis and exhibited higher tolerance to high-temperature stress than the wild-type strain. We ident

SUBMITTER: Isogai S 

PROVIDER: S-EPMC8426250 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature