Precision vibratome for high-speed ultrathin biotissue cutting and organ-wide imaging.

ABSTRACT: Cutting tissues into ultrathin slices is highly desired in sectioning-based organ-wide imaging. However, it is difficult to perform tissue cutting at a high speed with excellent quality. Here, we design a precision vibratome based on a paired double parallelogram flexure, which enables a vibrating blade to move strictly along a straight line. Meanwhile, we develop a high-speed cutting method that does not compromise cutting quality, which the vibratome operated at a high frequency mode. The characterized parasitic motion errors of a 180-Hz vibratome were less than 300 nm. It achieved a cutting speed six times that of an 85-Hz vibratome with acceptable quality. The capacity of the vibratome was investigated by organ-wide imaging, and the results revealed that it can be adapted in different

SUBMITTER: Li Y 

PROVIDER: S-EPMC8426277 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature