Smoking shifts human small airway epithelium club cells toward a lesser differentiated population.

ABSTRACT: The club cell, a small airway epithelial (SAE) cell, plays a central role in human lung host defense. We hypothesized that subpopulations of club cells with distinct functions may exist. The SAE of healthy nonsmokers and healthy cigarette smokers were evaluated by single-cell RNA sequencing, and unsupervised clustering revealed subpopulations of SCGCB1A1⁺KRT5^loMUC5AC^- club cells. Club cell heterogeneity was supported by evaluations of SAE tissue sections, brushed SAE cells, and in vitro air-liquid interface cultures. Three subpopulations included: (1) progenitor; (2) proliferating; and (3) effector club cells. The progenitor club cell population expressed high levels of mitochondrial, ribosomal proteins, and KRT5 relative to other club cell populations and

SUBMITTER: Rostami MR 

PROVIDER: S-EPMC8426481 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature