Exploring the Druggability of Conserved RNA Regulatory Elements in the SARS-CoV-2 Genome.

ABSTRACT: SARS-CoV-2 contains a positive single-stranded RNA genome of approximately 30 000 nucleotides. Within this genome, 15 RNA elements were identified as conserved between SARS-CoV and SARS-CoV-2. By nuclear magnetic resonance (NMR) spectroscopy, we previously determined that these elements fold independently, in line with data from in vivo and ex-vivo structural probing experiments. These elements contain non-base-paired regions that potentially harbor ligand-binding pockets. Here, we performed an NMR-based screening of a poised fragment library of 768 compounds for binding to these RNAs, employing three different ¹ H-based 1D NMR binding assays. The screening identified common as well as RNA-element specific hits. The results allow selection of the most promising of the 15 RNA ele

SUBMITTER: Sreeramulu S 

PROVIDER: S-EPMC8426693 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature