Unknown

Dataset Information

0

Discovery and Characterization of Spike N-Terminal Domain-Binding Aptamers for Rapid SARS-CoV-2 Detection.

ABSTRACT: The coronavirus disease 2019 (COVID-19) pandemic has devastated families and disrupted healthcare, economies and societies across the globe. Molecular recognition agents that are specific for distinct viral proteins are critical components for rapid diagnostics and targeted therapeutics. In this work, we demonstrate the selection of novel DNA aptamers that bind to the SARS-CoV-2 spike glycoprotein with high specificity and affinity (<80 nM). Through binding assays and high resolution cryo-EM, we demonstrate that SNAP1 (SARS-CoV-2 spike protein N-terminal domain-binding aptamer 1) binds to the S N-terminal domain. We applied SNAP1 in lateral flow assays (LFAs) and ELISAs to detect UV-inactivated SARS-CoV-2 at concentrations as low as 5×105  copies mL-1 . SNAP1 is therefore a promising molecular tool for SARS-CoV-2 diagnostics.

SUBMITTER: Kacherovsky N 

PROVIDER: S-EPMC8426805 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Discovery and Characterization of Spike N-Terminal Domain-Binding Aptamers for Rapid SARS-CoV-2 Detection.

Kacherovsky Nataly N   Yang Lucy F LF   Dang Ha V HV   Cheng Emmeline L EL   Cardle Ian I II   Walls Alexandra C AC   McCallum Matthew M   Sellers Drew L DL   DiMaio Frank F   Salipante Stephen J SJ   Corti Davide D   Veesler David D   Pun Suzie H SH  

Angewandte Chemie (International ed. in English) 20210818 39

The coronavirus disease 2019 (COVID-19) pandemic has devastated families and disrupted healthcare, economies and societies across the globe. Molecular recognition agents that are specific for distinct viral proteins are critical components for rapid diagnostics and targeted therapeutics. In this work, we demonstrate the selection of novel DNA aptamers that bind to the SARS-CoV-2 spike glycoprotein with high specificity and affinity (<80 nM). Through binding assays and high resolution cryo-EM, we  ...[more]

Similar Datasets

Unknown Discovery of Aptamers Targeting the Receptor-Binding Domain of the SARS-CoV-2 Spike Glycoprotein.
| S-EPMC7336720 | biostudies-literature
Unknown DNA Aptamers Block the Receptor Binding Domain at the Spike Protein of SARS-CoV-2.
| S-EPMC8397481 | biostudies-literature
Unknown Evolutionary remodelling of N-terminal domain loops fine-tunes SARS-CoV-2 spike.
| S-SCDT-EMBOR-2021-54322V1 | biostudies-other
Unknown Nucleocapsid protein binding DNA aptamers for detection of SARS-COV-2.
| S-EPMC10223630 | biostudies-literature
Unknown Detection of breastmilk antibodies targeting SARS-CoV-2 nucleocapsid, spike and receptor-binding-domain antigens.
| S-EPMC7782901 | biostudies-literature
Unknown Rapid Development of SARS-CoV-2 Spike Protein Receptor-Binding Domain Self-Assembled Nanoparticle Vaccine Candidates.
| S-EPMC7839421 | biostudies-literature
Unknown Two Receptor Binding Strategy of SARS-CoV-2 Is Mediated by Both the N-Terminal and Receptor-Binding Spike Domain.
| S-EPMC10801686 | biostudies-literature
Unknown Evidence of a putative glycosaminoglycan binding site on the glycosylated SARS-CoV-2 spike protein N-terminal domain.
| S-EPMC8093007 | biostudies-literature
Unknown Identification of possible binding modes of SARS-CoV-2 spike <i>N</i>-terminal domain for ganglioside GM1.
| S-EPMC9744490 | biostudies-literature
Unknown Evolutionary remodelling of N-terminal domain loops fine-tunes SARS-CoV-2 spike.
| S-EPMC9535765 | biostudies-literature