Enhancing the Stability of COVID-19 Serological Assay through Metal-Organic Framework Encapsulation.

ABSTRACT: Enzyme-linked immunosorbent assay is widely utilized in serologic assays, including COVID-19, for the detection and quantification of antibodies against SARS-CoV-2. However, due to the limited stability of the diagnostic reagents (e.g., antigens serving as biorecognition elements) and biospecimens, temperature-controlled storage and handling conditions are critical. This limitation among others makes biodiagnostics in resource-limited settings, where refrigeration and electricity are inaccessible or unreliable, particularly challenging. In this work, metal-organic framework encapsulation is demonstrated as a simple and effective method to preserve the conformational epitopes of antigens immobilized on microtiter plate under non-refrigerated storage conditions. It is demonstrated that in si

SUBMITTER: Wang Y 

PROVIDER: S-EPMC8427112 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature