Single-cell analysis at the protein level delineates intracellular signaling dynamic during hematopoiesis.

ABSTRACT:

Background

Hematopoietic stem and progenitor cell (HSPC) subsets in mice have previously been studied using cell surface markers, and more recently single-cell technologies. The recent revolution of single-cell analysis is substantially transforming our understanding of hematopoiesis, confirming the substantial heterogeneity of cells composing the hematopoietic system. While dynamic molecular changes at the DNA/RNA level underlying hematopoiesis have been extensively explored, a broad understanding of single-cell heterogeneity in hematopoietic signaling programs and landscapes, studied at protein level and reflecting post-transcriptional processing, is still lacking. Here, we accurately quantified the intracellular levels of 9 phosphorylated and 2 functional proteins at the single-

SUBMITTER: Wang J 

PROVIDER: S-EPMC8428103 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature