TRIB3 regulates FSHR expression in human granulosa cells under high levels of free fatty acids.

ABSTRACT:

Background

Granulosa cells (GCs) in cumulus oophorus highly express follicle stimulating hormone receptor (FSHR), which is the most important mediator of both estradiol synthesis and oocyte maturation. Obese women have elevated free fatty acids (FFAs) levels in their follicular fluids and decreased FSHR expression in GCs, which is related to an altered protein kinase B/glycogen synthase kinase 3β (Akt/GSK3β) signaling pathway. Such FFA increases accompany 3-fold rises in pseudokinase 3 (TRIB3) expression and reduce the Akt phosphorylation status in both the human liver and in insulinoma cell lines. Therefore, in a high FFA environment, we determined if TRIB3 mediates regulation of FSHR via the Akt/GSK3β signaling pathway in human GCs.

Methods

GCs from women undergoing in vit

SUBMITTER: Wang N 

PROVIDER: S-EPMC8428109 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature