Impairment of Autophagic Flux Participates in the Antitumor Effects of TAT-Cx43_266-283 in Glioblastoma Stem Cells.

ABSTRACT: Autophagy is a physiological process by which various damaged or non-essential cytosolic components are recycled, contributing to cell survival under stress conditions. In cancer, autophagy can have antitumor or protumor effects depending on the developmental stage. Here, we use Western blotting, immunochemistry, and transmission electron microscopy to demonstrate that the antitumor peptide TAT-Cx43_266-283, a c-Src inhibitor, blocks autophagic flux in glioblastoma stem cells (GSCs) under basal and nutrient-deprived conditions. Upon nutrient deprivation, GSCs acquired a dormant-like phenotype that was disrupted by inhibition of autophagy with TAT-Cx43_266-283 or chloroquine (a classic autophagy inhibitor), leading to GSC death. Remarkably, dasatinib, a clinically availa

SUBMITTER: Pelaz SG 

PROVIDER: S-EPMC8428230 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature