CRISPR/Cas9-based discovery of maize transcription factors regulating male sterility and their functional conservation in plants.

ABSTRACT: Identifying genic male-sterility (GMS) genes and elucidating their roles are important to unveil plant male reproduction and promote their application in crop breeding. However, compared with Arabidopsis and rice, relatively fewer maize GMS genes have been discovered and little is known about their regulatory pathways underlying anther and pollen development. Here, by sequencing and analysing anther transcriptomes at 11 developmental stages in maize B73, Zheng58 and M6007 inbred lines, 1100 transcription factor (TF) genes were identified to be stably differentially expressed among different developmental stages. Among them, 14 maize TF genes (9 types belonging to five TF families) were selected and performed CRISPR/Cas9-mediated gene mutagenesis, and then, 12 genes in eight types, includin

SUBMITTER: Jiang Y 

PROVIDER: S-EPMC8428822 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature