Scaled, high fidelity electrophysiological, morphological, and transcriptomic cell characterization.

ABSTRACT: The Patch-seq approach is a powerful variation of the patch-clamp technique that allows for the combined electrophysiological, morphological, and transcriptomic characterization of individual neurons. To generate Patch-seq datasets at scale, we identified and refined key factors that contribute to the efficient collection of high-quality data. We developed patch-clamp electrophysiology software with analysis functions specifically designed to automate acquisition with online quality control. We recognized the importance of extracting the nucleus for transcriptomic success and maximizing membrane integrity during nucleus extraction for morphology success. The protocol is generalizable to different species and brain regions, as demonstrated by capturing multimodal data from human and macaque

SUBMITTER: Lee BR 

PROVIDER: S-EPMC8428855 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature