Interruption of endolysosomal trafficking leads to stroke brain injury.

ABSTRACT:

Background and purpose

Dysfunction of the endolysosomal system can cause cell death. A key molecule for controlling the endolysosomal trafficking activities is the N-ethylmaleimide-sensitive factor (NSF) ATPase. This study investigates the cascades of NSF ATPase inactivation events, endolysosomal damage, cathepsin release, and neuronal death after focal brain ischemia.

Methods

A total of 62 rats were used in this study. They were subjected to sham surgery or 2 h of focal brain ischemia followed by 1, 4, and 24 h of reperfusion. Confocal microscopy and Western blot analysis were utilized to analyze the levels, redistribution, and co-localization of key proteins of the Golgi apparatus, late endosomes, endolysosomes, and lysosomes. Light and electron microscopy were used to exa

SUBMITTER: Yuan D 

PROVIDER: S-EPMC8429234 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature