Characterization and allogeneic transplantation of a novel transgenic cone-rich donor mouse line.

ABSTRACT:

Objectives

Cone photoreceptor transplantation is a potential treatment for macular diseases. The optimal conditions for cone transplantation are poorly understood, partly because of the scarcity of cones in donor mice. To facilitate allogeneic cone photoreceptor transplantation studies in mice, we aimed to create and characterize a donor mouse model containing a cone-rich retina with a cone-specific enhanced green fluorescent protein (EGFP) reporter.

Methods

We generated OPN1LW-EGFP/NRL^-/- mice by crossing NRL^-/- and OPN1LW-EGFP mice. We characterized the anatomical phenotype of OPN1LW-EGFP/NRL^-/- mice using multimodal confocal scanning laser ophthalmoscopy (cSLO) imaging, immunohistology, and transmission electron microscopy. We evaluated re

SUBMITTER: Liu YV 

PROVIDER: S-EPMC8429259 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature