In-house reverse transcriptase polymerase chain reaction for detection of SARS-CoV-2 with increased sensitivity.

ABSTRACT: As the COVID-19 infection continues to ravage the world, the advent of an efficient as well as the economization of the existing RT-PCR based detection assay essentially can become a blessing in these testing times and significantly help in the management of the pandemic. This study demonstrated an innovative and rapid corroboration of COVID-19 test based on innovative multiplex PCR. An assessment of optimal PCR conditions to simultaneously amplify the SARS-CoV-2 genes E, S and RdRp has been made by fast-conventional and HRM coupled multiplex real-time PCR using the same sets of primers. All variables of practical value were studied by amplifying known target-sequences from ten-fold dilutions of archived positive samples of COVID-19 disease. The multiplexing with newly designed E, S and Rd

SUBMITTER: Kalita MJ 

PROVIDER: S-EPMC8429455 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature