Comparative effects of alive and pasteurized Akkermansia muciniphila on normal diet-fed mice.

ABSTRACT: Recently, Akkermansia muciniphila an anaerobic member of the gut microbiota, has been proposed as a next-generation probiotic. The aim of this study was evaluation of the effect of alive and pasteurized A. muciniphila on health status, intestinal integrity, immune response, lipid metabolism, and gut microbial composition in normal-diet fed mice as well as direct effects of the bacterium on Caco-2 cell line. A total of 30 mice were distributed into three different groups, control, alive, and pasteurized A. muciniphila-treated group. After acclimation, control and treatment groups were administrated with PBS and 10⁹ CFU/200µL of bacterial suspension for 5 weeks, respectively. Besides, Caco-2 separately exposed to alive, pasteurized A. muciniphila and PBS for 24 h. The results show

SUBMITTER: Ashrafian F 

PROVIDER: S-EPMC8429653 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature