Silencing of PFKFB3 protects podocytes against high glucose‑induced injury by inducing autophagy.

ABSTRACT: Diabetic nephropathy (DN) is a diabetic complication that threatens the health of patients with diabetes. In addition, podocyte injury can lead to the occurrence of DN. The protein 6‑phosphofructo‑2‑kinase/fructose‑2,6-biphosphatase 3 (PFKFB3) may be associated with diabetes; however, the effects of PFKFB3 knockdown by small interfering (si)RNA on the growth of podocytes remains unknown. To investigate the mechanism by which PFKFB3 mediates podocyte injury, MPC5 mouse podocyte cells were treated with high‑glucose (HG), and cell viability and apoptosis were examined by Cell Counting Kit‑8 assay and flow cytometry, respectively. In addition, the expression of autophagy‑related proteins were measured using western blot analysis and immunofluorescence staining. Cell migration was investigated

SUBMITTER: Zhu Z 

PROVIDER: S-EPMC8430303 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature