O‑GlcNAcylation contributes to intermittent hypoxia‑associated vascular dysfunction via modulation of MAPKs but not CaMKII pathways.

ABSTRACT: Intermittent hypoxia (IH) leads to vascular dysfunction, and O‑linked‑β‑N‑acetylglucosamine (O‑GlcNAc)ylation may regulate vascular reactivity through the modulation of intracellular signaling. The present study hypothesized that O‑GlcNAc modifications contributed to the vascular effects of acute IH (AIH) and chronic IH (CIH) through the MAPK and Ca2+/calmodulin‑dependent kinase II (CaMKII) pathways. Rat aortic and mesenteric segments were incubated with DMSO, O‑GlcNAcase (OGA) or O‑GlcNAc transferase (OGT) inhibitor under either normoxic or AIH conditions for 3 h, and arterial function was then assessed. Meanwhile, arteries isolated from control and CIH rats were exposed to 3 h of incubation under normoxic conditions using DMSO, OGA or OGT as an inhibitor, before assessing arterial reacti

SUBMITTER: Guo X 

PROVIDER: S-EPMC8430318 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature