Multilevel Approach for the Treatment of Giardiasis by Targeting Arginine Deiminase.

ABSTRACT: Giardiasis represents a latent problem in public health due to the exceptionally pathogenic strategies of the parasite Giardia lamblia for evading the human immune system. Strains resistant to first-line drugs are also a challenge. Therefore, new antigiardial therapies are urgently needed. Here, we tested giardial arginine deiminase (GlADI) as a target against giardiasis. GlADI belongs to an essential pathway in Giardia for the synthesis of ATP, which is absent in humans. In silico docking with six thiol-reactive compounds was performed; four of which are approved drugs for humans. Recombinant GlADI was used in enzyme inhibition assays, and computational in silico predictions and spectroscopic studies were applied to follow the enzyme's structural disturbance and identify pos

SUBMITTER: Fernandez-Lainez C 

PROVIDER: S-EPMC8431557 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature