Application of CD54 in diagnosing bone marrow involvement by using flow cytometry in patients with diffuse large B-cell lymphoma.

ABSTRACT:

Background

Flow cytometry plays a key role in detecting bone marrow (BM) involvement in patients with diffuse large B-cell lymphoma (DLBCL). To improve its detection sensitivity, we need to explore novel markers. In this study, we detected the expression CD54 on lymphoma cells in BM specimens from DLBCL patients and clarified its diagnostic significance in BM involvement by DLBCL.

Methods

We collected BM specimens from 76 patients with DLBCL (germinal center B-cell (GCB) = 25, non-GCB = 51) and 10 control patients without lymphoma. We detected and compared the expression of CD54 on lymphoma cells and normal mature B cells by using 10-color panels.

Results

Normal plasma cells expressed a higher level of CD54 as compared with hematogones (p < 0.05) and normal mature B c

SUBMITTER: Wang W 

PROVIDER: S-EPMC8431857 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature