Improving Mobilization of Foreign DNA into Zymomonas mobilis Strain ZM4 by Removal of Multiple Restriction Systems.

ABSTRACT: Zymomonas mobilis has emerged as a promising candidate for production of high-value bioproducts from plant biomass. However, a major limitation in equipping Z. mobilis with novel pathways to achieve this goal is restriction of heterologous DNA. Here, we characterized the contribution of several defense systems of Z. mobilis strain ZM4 to impeding heterologous gene transfer from an Escherichia coli donor. Bioinformatic analysis revealed that Z. mobilis ZM4 encodes a previously described mrr-like type IV restriction modification (RM) system, a type I-F CRISPR system, a chromosomal type I RM system (hsdMS_c), and a previously uncharacterized type I RM system, located on an endogenous plasmid (hsdRMS_p). The DNA recognition motif of HsdRMS_p w

SUBMITTER: Lal PB 

PROVIDER: S-EPMC8432527 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature