Clinical impact of serum exosomal microRNA in liver fibrosis.

ABSTRACT:

Background/aim

We investigated alterations in the expression of serum exosomal miRNAs with the progression of liver fibrosis and evaluated their clinical applicability as biomarkers.

Methods

This study prospectively enrolled 71 patients who underwent liver biopsy at an academic hospital in Korea. Exosomes were extracted from serum samples, followed by next-generation sequencing (NGS) of miRNAs and targeted real-time quantitative polymerase chain reaction. A model was derived to discriminate advanced fibrosis based on miRNA levels and the performance of this model was evaluated. Validation of the effect of miRNA on liver fibrosis in vitro was followed.

Results

NGS data revealed that exosomal miR-660-5p, miR-125a-5p, and miR-122 expression were changed significantly wit

SUBMITTER: Chang Y 

PROVIDER: S-EPMC8432846 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature