Automated phenotyping of Caenorhabditis elegans embryos with a high-throughput-screening microfluidic platform.

ABSTRACT: The nematode Caenorhabditis elegans has been extensively used as a model multicellular organism to study the influence of osmotic stress conditions and the toxicity of chemical compounds on developmental and motility-associated phenotypes. However, the several-day culture of nematodes needed for such studies has caused researchers to explore alternatives. In particular, C. elegans embryos, due to their shorter developmental time and immobile nature, could be exploited for this purpose, although usually their harvesting and handling is tedious. Here, we present a multiplexed, high-throughput and automated embryo phenotyping microfluidic approach to observe C. elegans embryogenesis after the application of different chemical compounds. After performing experiments with u

SUBMITTER: Baris Atakan H 

PROVIDER: S-EPMC8433184 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature